Chelsea look set to play at Wembley from 2018 after the £500million redevelopment of Stamford Bridge was given the green light.
The Blues were granted planning permission by Hammersmith and Fulham council in January to build a new 60,000 capacity stadium.
Those plans were rubber-stamped by London Mayor Sadiq Khan on Monday morning , meaning Chelsea will have to find a new home for three years while work is carried out.
Wembley and Twickenham had been identified, although residents close to the home of rugby have opposed Chelsea moving due to fears over increased traffic and anti-social behaviour.
RFU chief Ian Ritchie has confirmed “there is no intention” to host matches at the home of rugby.
A letter from Twickenham MP Dr Tania Mathias to Ritchie last week, read: “Following the decision by Hammersmith and Fulham council to approve plans for Chelsea’s new stadium, I am writing to you to re-state my position that I do not support Chelsea FC being based, temporarily or otherwise, at Twickenham.
“My opposition to more non-rugby fixtures at Twickenham is based on concerns regarding increased problems for local residents such as match-day traffic, an increase in litter, public urination and other anti-social behaviour.
“I would appreciate reassurance from yourself that my fears are unfounded, as hosting regular football matches at the RFU Stadium is clearly the wrong decision for Twickenham.”
Wembley now looks set to host Chelsea matches for three seasons from 2018 onwards as work takes place at Stamford Bridge.
Khan said: “I’m confident this new stadium will be a jewel in London’s sporting crown and will attract visitors and football fans from around the world.”
Tottenham will play at Wembley next term while their ground is rebuilt but Spurs expect to be in their new home for the 2018-19 season.